文章基本信息

标题：Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
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作者：Hemanth K.N. Reddy ; Chun Hong Yoon ; Andrew Aquila 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2017
卷号：4
DOI：10.1038/sdata.2017.79
语种：English
出版社：Nature Publishing Group
摘要：Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65鈥?0鈥塶m, which is considerably smaller than the previously reported ~600鈥塶m diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.