文章基本信息

标题：The effect of 16S rRNA region choice on bacterial community metabarcoding results
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作者：Yu. S. Bukin ; Yu. P. Galachyants ; I. V. Morozov 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2019
卷号：6
DOI：10.1038/sdata.2019.7
语种：English
出版社：Nature Publishing Group
摘要：In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.