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标题：Map of physical interactions between extracellular domains of Arabidopsis leucine-rich repeat receptor kinases
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作者：G. Adam Mott ; Elwira Smakowska-Luzan ; Asher Pasha 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2019
卷号：6
DOI：10.1038/sdata.2019.25
语种：English
出版社：Nature Publishing Group
摘要：Plants use surface receptors to perceive information about many aspects of their local environment. These receptors physically interact to form both steady state and signalling competent complexes. The signalling events downstream of receptor activation impact both plant developmental and immune responses. Here, we present a comprehensive study of the physical interactions between the extracellular domains of leucine-rich repeat receptor kinases (LRR-RKs) in Arabidopsis. Using a sensitized assay, we tested reciprocal interactions among 200 of the 225 Arabidopsis LRR-RKs for a total search space of 40,000 interactions. Applying a stringent statistical cut-off and requiring that interactions performed well in both bait-prey and prey-bait orientations resulted in a high-confidence set of 567 bidirectional interactions. Additionally, we identified a total of 2,586 unidirectional interactions, which passed our stringent statistical cut-off in only one orientation. These datasets will guide further investigation into the regulatory roles of LRR-RKs in plant developmental and immune signalling decisions.