摘要:AbstractBrassica junceais an allopolyploid originating from the interspecific hybridization betweenBrassica rapaandBrassica nigra, which is of multiple usage as a vegetable, oilseed and condiment worldwide.Both vernalization and non-vernalization under long-day photoperiod can promote floral transition inB. junceasuggesting merged flowering pathways of its ancestors and better environmental adaptability.We identified genome-wide flowering regulatory genes inB. juncea, which include 84 and 79 genes from A and B sub-genomes, respectively. Ka/Ks analysis revealed a purification effect on both photoperiod and vernalization flowering regulation pathways during evolution. Expression profile of those genes during long-day and vernalization treatments suggestedBjuACO4, BjuAFT1, BjuBFT4, BjuASOC1andBjuASOC4may be the major functional copies ofB. junceaflowering regulation. Further functional studies aboutBjuCOsshowed three days delayed flowering time inBjuACO4orBjuBCO3silenced plants. Increased transcription of allBjuFLCsinBjuACO4orBjuBCO3silenced plants suggested interactions between photoperiod and vernalization pathways governing flowering time. Our findings provided flowering regulating networks in allopolyploidB. juncea.