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标题：Phytochemical Screening, Evaluation of Antioxidantand Anti-sickling Activities of Two Polar Extracts ofCombretum glutinosum Leaves. Perr. ex DC
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作者：Cheikh Sall ; Samba Fama Ndoye ; Mbaye Diaw Dioum 等
期刊名称：Current Journal of Applied Science and Technology
印刷版ISSN：2457-1024
出版年度：2017
卷号：19
期号：3
页码：1-11
语种：English
出版社：Sciencedomain International
摘要：Sickle cell disease is a major public health problem in Africa and many other areas across the world. In Senegal, a lot of plants are proposed by traditional healers to manage the sickle cell disease, among them Combretum glutinosum. Antisickling activity of Combretum glutinosum leaves extracts was studied in this work. Methanolic and ethyl acetate extracts of Combretum glutinosum leaves were evaluated on SS sickles type to determine their anti-sickling potential. Antiradical properties of methanolic extract were evaluated using the DPPH radical as oxidant. Total phenolic content of the methanol extract was determined. Phytochemical screening of the crude extract of methanol revealed the presence of tannins, saponins, phenols and flavonoids. Results show a good antisickling effect of extracts with a maximum antisickling revers of 81% and 69% respectively, of the methanol and ethyl acetate extracts at 10 mg/mL versus 67% for arginine at 120 min of incubation. The measured IC50 were 0.65 and 0.163 for respectively the methanol extract and ascorbic acid. Antiradical powers 0.155 and 0.62 respectively for methanol extract and ascorbic acid were calculated from the effective concentrations. The results of this study confirm the traditional use of Combretum glutinosum leaves in the management of sickle cell disease.
关键词：Sickle cell disease;oxidative stress;medicinal plant;Combretum glutinosum;antisicklingactivity;antioxidant activity