摘要:[Objective] The aim of study is to research the treatment of urban sewage by the semi natural wetland method in the high connectivity water system of the surface water and surface water in the inland waterway. [Methods] Microbial diversity and community structure in the surface water of eight samples on the Xiantao artificial wetland were investigated by using the Illumina Miseq sequencing technique and the resulting microbial data were statistically analyzed in combination with environmental variabl. [Results] The results showed that COD concentration had a very significant positive correlation with total microbial communities (r = 0.700, P < 0.01), and TP, and TN were significantly positively correlated (r = 0.509, 0.517 and 0.508, P < 0.05). In addition, COD concentration was positively correlated with abundant groups (r = 0.488, P < 0.05), and TP, and TN were significantly positively correlated (r = 0.537, 0.356 and 0.360, P < 0.05). For rare groups, they may be more sensitive to the environment than their abundant groups. [Conclusion] For rare taxa, it is likely to be more sensitive to the environment than their rich taxa. The relative abundance of rare taxa of samples collected when buried deep 5 m is greater than that of surface water. Therefore, the environmental conditions of microbial responses (rare taxa) should be carefully evaluated in the future.