文章基本信息

标题：Antimicrobial photodynamic inactivation of fungal biofilm using amino functionalized mesoporus silica-rose bengal nanoconjugate against Candida albicans
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作者：Parasuraman Paramanantham ; Asha P Antony ; S.B. Sruthil Lal 等
期刊名称：Scientific African
印刷版ISSN：2468-2276
出版年度：2018
卷号：1
页码：1-17
DOI：10.1016/j.sciaf.2018.e00007
语种：English
出版社：Elsevier
摘要：Highlights•The study emphasizes the enhanced aPDT activity of prepared MSN-RB nanoconjugate against planktonic cells and biofilm ofC. albicans.•Antimicrobial activity of MSN-RB nanoconjugate is more efficient againstC. albicans.•The mechanism of action of aPDT was studied further and the results were in good agreement with previous literature.•The nanoconjugate of present study can be used to eradicate the growth and virulence ofC. albicanssignificantly.AbstractCandida albicansis an opportunistic fungal pathogen that causes both superficial and systemic infection and an important candidate that contribute to high morbidity and mortality rates in immunocompromised patients. The ability ofC. albicansto switch from yeast to filamentous form and thereby forming biofilms make them resistant to most of the antifungal drugs available today. Thus the development of more effective antifungal drugs are essential and crucial at this point of time. Antimicrobial photodynamic therapy is an alternative modality to treat such biofilm forming resistant strains. This study aims to investigate the enhanced efficiency of newly synthesized MSN-RB conjugate as an antimicrobial photosensitizer for antimicrobial photodynamic therapy againstC. albicans. Functionalization of MSN with amino groups was performed to increase the dye loading capacity. Conjugation process of MSN-RB was confirmed using different techniques including UV–Vis spectroscopy, Fluorescent spectroscopy and FTIR analysis. A low power green laser 50 mW irradiation was applied (5 min) for activation of MSN-RB conjugate and RB againstC. albicansbiofilm and planktonic cell. The comparative study of MSN-RB conjugate and free RB on aPDT was evaluated using standard experimental procedures. Antibiofilm efficacy was determined using biofilm inhibition assay, cell viability, EPS quantification and CLSM studies. The results revealed that MSN-RB conjugate has a significant antimicrobial activity (88.62 ± 3.4%) and antibiofilm effect onC. albicanswhen compared to free dye after light irradiation. The MSN-RB conjugate based aPDT can be employed effectively in treatment ofC. albicansinfections.Graphical abstractDisplay Omitted
关键词：KeywordsAntimicrobial photodynamic therapyMesoporus silica nanoparticlesAmino functionalizationConjugationLipid peroxidationAnti-biofilm activity