Objectives: This study examined the psychometric properties of the Brief Fear of Negative Evaluation Scale (FNES-B) in a nonclinical, student sample. Method: 325 students (143 male and 182 female) who were selected using randomized multi-stage sampling method responded to FNES-B Scale and Academic Expectations Stress Inventory (AESI). In this research, the confirmatory factor analysis was used to determine the validity of FNES-B, and Cronbach’s alpha was used to determine its internal consistency. Results: Confirmatory factor analysis revealed that while the unidimensional model of Brief Fear of Negative Evaluation Scale had an acceptable fit to the data, the two-factor model of Brief Fear of Negative Evaluation Scale (including positive and reverse scored items) demonstrated a superior fit to the data. The data also supported the reliability of FNES-B scores. In other words internal consistency was good for the positive scored factor (=α0.87) and for the full FNES-B scale (=α0.84) and acceptable for the reverse scored factor (=α0.47). FNES-B scores were significantly correlated with the AESI (p<0.001). Female students scored higher in FNES-B scores in comparison with male students. Conclusion: FNES-B is a valid and reliable measurement instrument for measuring fear of negative evaluation in students.