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标题：Molecular detection of integron and antimicrobial resistance genes in multidrug resistant Salmonella isolated from poultry, calves and human in Beni-Suef governorate, Egypt
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作者：Sahar A. Abdel Aziz ; Gihan K. Abdel-Latef ; Salama A.S. Shany 等
期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2018
卷号：7
期号：4
页码：535-542
DOI：10.1016/j.bjbas.2018.06.005
语种：English
出版社：Elsevier
摘要：Highlights•All serotypes of isolatedSalmonellaexhibit extremely high rate of multiple drug resistance indexed to all antibiotics used.•PCR confirmed the results of the antibiogram.•Multidrug resistantSalmonellaeare more prevalent in poultry flocks and this represents a major threat to poultry handlers.•Limitation of the unnecessary application of antibiotics for poultry and farm animals is recommended.AbstractAntibiotic resistantSalmonellarepresent a public health threat worldwide. The current study was delineated to determineSalmonellaspp. prevalent in poultry droppings (N: 14 flocks), calves feces (N: 90) and human stool samples (N: 80) from live-stock contacts as well as poultry handler in Beni-Suef governorate, Egypt. Phenotypic and genotypic screening for the presence of antimicrobial resistance was applied. Isolation rates were 57.1%, 5.6% and 2.5% for poultry, calves and humans, respectively. S. Megherafelt. S. Kentucky, S. Amsterdam, S. Nigeria, S. Paratyphi: B2, S. Alamo, S. MadrasandS. Haifawere the predominant serovars in poultry flocks under investigation. The predominant serovar from calves’ samples wasS. KentuckywhileS. InfantisandS. Paratyphi: B2were detected in human samples. The results of antimicrobial disc diffusion revealed the presence of multiple drug resistance (MDR) in100% ofSalmonellaisolates. PCR confirmed the existence of class 1 integron and the following gene cassettes in the resistant strains,blaTEMandblaSHVfor β-lactams,aadA2for aminoglycosides (streptomycin),tetAandtetBfor tetracycline,sullfor Sulfamethoxazole,dfrAfor trimethoprim,floRfor chloramphenicol andmphAfor macrolides.
关键词：Salmonella;Calves;Poultry;Antibiotic resistance;Integron class 1;Egypt