文章基本信息

标题：A new method for acquiring images of meiobenthic images using the FlowCAM
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作者：Tomo Kitahashi ; Hiromi Kayama Watanabe ; Masashi Tsuchiya 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2018
卷号：5
页码：1330-1335
DOI：10.1016/j.mex.2018.10.012
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractThe purpose of this study was to develop a new method for investigating sediment-inhabiting meiobenthos using the Flow Cytometer And Microscope (FlowCAM). Meiobenthos are widely recognized as a useful indicator for assessing the effects of anthropogenic and natural disturbances in both shallow and deep ocean ecosystems. These small benthic invertebrates are traditionally investigated by individually counting and identifying specimens under a microscope, which is labor intensive and time consuming. However, FlowCAM, which was originally developed to semiautomatically analyze microplankton, has the potential to resolve these challenges. Meiobenthic specimens were extracted from sediment using the centrifugal separation method and were then pipetted into the FlowCAM system and imaged. The images were then used to classify and count the specimens at high taxonomic levels to verify the effectiveness of this method compared with traditional methods. We found that FlowCAM system:•Enabled sufficient meiobenthic images to be obtained to allow the identification and classification of specimens at high taxonomic levels.•Obtained comparable numbers of individuals to traditional methods.•Has the potential to rapidly process large the volumes of meiobenthos samples that are required when monitoring seasonal and spatial variation in ocean ecosystems and conducting long-term environmental impact assessments.
关键词：Meiobenthos;FlowCAM;Environmental monitoring;Seasonal variation