文章基本信息

标题：Comparative analysis and innovation of a simple and rapid method for high-quality RNA and DNA extraction of kiwifruit
本地全文：下载
作者：Mansour Afshar-Mohammadian ; Mohammad Hossein Rezadoost ; Seyyed Fatemeh Fallah 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2018
卷号：5
页码：352-361
DOI：10.1016/j.mex.2018.03.008
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractRNA and DNA extraction is a requirement for the study of gene expression and has an increasingly important role in genetic studies of all fleshy fruits. RNA and DNA extraction is difficult in kiwifruit due to the significant amount of polysaccharides and polyphenols compounds. So far, no commercial kit has been developed specifically for high-quality RNA and DNA extraction in kiwifruit and the common protocols for RNA extraction have poor yields. This study developed a new protocol for high quality RNA extraction inActinidia deliciosa. According to the results, the average yield of RNA extraction of fruit and leaf ofA. deliciosawas ∼2180.7 ng/μl (∼545.175 μg/g FW) and ∼3424.9 ng/μl (∼856.225 μg/g FW), respectively with A260/A280between 1.95 to 2.07 and A260/A230higher than 2 indicating high RNA purity. While the averages yield of RNA extraction using previous methods from kiwifruit and leaf was 23 μg/g FW and 527 μg/g FW, respectively. Also, the average yields of genomic DNA from kiwifruit ranged from 52 to 98 ng/μl with A260/A230between 0.60 to 1.64 and A260/A280between 1.40 to 1.48. To our knowledge, this is the first report of a highly efficient and rapid method of RNA and DNA extraction in kiwifruit which can be used for a broad spectrum of the all fleshy fruits.
关键词：RNA and DNA extraction;RT-qPCR;Kiwifruit;Polyphenols;Polysaccharides