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标题：Total starch in animal feeds and silages based on the chromatographic determination of glucose
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作者：Marcela María Salazar Murillo ; Fabio Granados-Chinchilla
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2018
卷号：5
页码：83-89
DOI：10.1016/j.mex.2018.01.009
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractStarch is an important nutrient in animal feed, and so its analysis is of considerable concern as it is one of the most relevant energy containing fractions. Method AOAC 996.11 was modified to exchange the enzymometric and colorimetric step full approach to a simpler HPLC amine-based column one. The method was optimized and validated for its application in animal feeds and silages.•We demonstrated that the method could be used for quality control for animal feeds and silages•We modified the final incubation time, the initial sample mass, the quantity of enzyme added and buffered, to pH 6.2, the medium to which α-amylase is added.•We applied a chromatographic analysis of the glucose that resulted from starch enzymatic hydrolysis, via a refractive index detector and amine-based chromatographic column.
关键词：Starch;Glucose;Enzymatic hydrolysis;Liquid chromatography;Refractive index detector;Feed;Silage