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标题：Isolation and characterization of bacteriocin producing Enterococcus casseliflavus and its antagonistic effect on Pseudomonas aeruginosa
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作者：M. Indira ; M. Indira ; T.C. Venkateswarulu 等
期刊名称：Karbala International Journal of Modern Science
印刷版ISSN：2405-609X
电子版ISSN：2405-609X
出版年度：2018
卷号：4
期号：4
页码：361-368
DOI：10.1016/j.kijoms.2018.09.002
语种：English
出版社：Elsevier
摘要：AbstractThe discovery of antimicrobial bacteriocin molecules with broad-spectrum activity against bacterial infections caused by spoilage microorganisms is vital for food safety. In this study, a potential bacteriocin-producing bacterium was isolated, screened and confirmed asEnterococcus casseliflavusMI001 by 16s rDNA sequencing. The bacteriocin peptide was purified and analysed by MALDI-TOF, which revealed the presence of a bacteriocin ABC transporter protein with a molecular mass of 22.53 kDa. The purified bacteriocin gave a final yield of 2.0%, with a specific activity of 15,000 AU/mg. The bacteriocin showed antagonistic activity againstPseudomonas aeruginosaand has potential as an antioxidant.
关键词：Bacteriocin;Probiotic;Enterococcus casseliflavus;Antimicrobial activity;Antioxidant activity