文章基本信息

标题：Increasing content of lipid in tropical microalgae Chlorella sorokiniana and Closterium sp. with variation of nitrogen content and extraction temperature
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作者：Amani Karima ; Mawar DS Silalahi ; Astri Rinanti 等
期刊名称：MATEC Web of Conferences
电子版ISSN：2261-236X
出版年度：2018
卷号：197
DOI：10.1051/matecconf/201819713019
语种：English
出版社：EDP Sciences
摘要：This research was aimed to obtain algae biofuels as an alternative energy which comes from tropical microalgae biomassChlorella sorokinianaandClosterium sp. The cultivation was performed at a controlled room in batch culturephotobioreactor, temperature 27°C, pH 6, aeration with air flow rate of 150 mL/sec, and light intensity at 2400 lux. These nutrient sources used artificial PHM with KNO3variation as a nitrogen source of 0 grams; 0.25 grams; 0.5 grams; 1 gram. The research involved microalgae lipid extraction withBlight & Dyermethod and also carried out support of alcohol, chloroform and distilled water (1:1:1) with optimum temperature 30oC. Oil yields that obtained were analyzed withGas Chromatography Mass Spectrometry(GC-MS) method, dry weight with a gravimetric method and cell density with spectrophotometry. The result showed that the reduction of KNO3materials (0,25 gr) can produce biomass and highest total lipid content at 0,41 g/L and 20,31 %/(w/w). The results showed that KNO3decreased the amount of biomass which was not significant but the total lipid content of microalgae was increased. Lipid content and fatty acids extracted from Blight & Dyer showed oil content that could potentially be the raw material of biodiesel.