文章基本信息

标题：Death patterns resulting from cell cycle-independent cell death. ⁎
本地全文：下载
作者：Dirke Imig ; Karsten Kuritz ; Nadine Pollak 等
期刊名称：IFAC PapersOnLine
印刷版ISSN：2405-8963
出版年度：2018
卷号：51
期号：19
页码：90-93
DOI：10.1016/j.ifacol.2018.09.028
语种：English
出版社：Elsevier
摘要：AbstractDrug-induced cell death on the one hand, and dynamics of the cell cycle on the other hand, represent two well-investigated biological principles. As the cell death machinery is highly regulated and depends on a large number of signaling proteins, a connection to the cell cycle is standing to reason. In recent studies, fluorescent cell cycle marker techniques have been combined with longterm microscopy approaches in order to shed light upon a possible cell cycle/cell death dependency for different death-inducing drugs. Here, we present a simple mechanistic model of the null hypothesis where a cell’s cycle does not influence cell death, and calculate death events in different phases of the cell cycle. In a first sample calculation, cells located according to a steady state distribution within the cell cycle are exposed to three different drugs. Dependent on the drug’s mode of action, different death patterns are observed. The second sample calculation considers the exposure of synchronized cell populations to one particular drug. Results show that an increase of cell death in G1can be obtained although cells are initially synchronized in different phases. We conclude that cumulated cell death in one phase can be explained by the stated null hypothesis. Last, the empirical mean and standard deviation are calculated for different sample sizes. Given experimental data, the presented results may help to find out whether the null hypothesis can be rejected.
关键词：KeywordsCell divisionCell deathCell cycle-dependenceSamplingFucci Cells