文章基本信息

标题：Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
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作者：Pieter Mestdagh ; Steve Lefever ; Pieter-Jan Volders 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2016
卷号：3
DOI：10.1038/sdata.2016.52
语种：English
出版社：Nature Publishing Group
摘要：Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression regulation. To study lncRNAs in cancer, we generated expression profiles for 1707 human lncRNAs in the NCI60 cancer cell line panel using a high-throughput nanowell RT-qPCR platform. We describe how qPCR assays were designed and validated and provide processed and normalized expression data for further analysis. Data quality is demonstrated by matching the lncRNA expression profiles with phenotypic and genomic characteristics of the cancer cell lines. This data set can be integrated with publicly available omics and pharmacological data sets to uncover novel associations between lncRNA expression and mRNA expression, miRNA expression, DNA copy number, protein coding gene mutation status or drug response