文章基本信息

标题：Serial femtosecond crystallography datasets from G protein-coupled receptors
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作者：Thomas A. White ; Anton Barty ; Wei Liu 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2016
卷号：3
DOI：10.1038/sdata.2016.57
语种：English
出版社：Nature Publishing Group
摘要：We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.