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  • 标题:Genome sequencing of a single tardigrade Hypsibius dujardini individual
  • 本地全文:下载
  • 作者:Kazuharu Arakawa ; Yuki Yoshida ; Masaru Tomita
  • 期刊名称:Scientific Data
  • 电子版ISSN:2052-4463
  • 出版年度:2016
  • 卷号:3
  • DOI:10.1038/sdata.2016.63
  • 语种:English
  • 出版社:Nature Publishing Group
  • 摘要:Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.
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