文章基本信息

标题：A novel method for extraction of a proteinous coagulant from Plantago ovata seeds for water treatment purposes
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作者：Bahman Ramavandi ; Seyedenayat Hashemi ; Raheleh Kafaei 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2015
卷号：2
页码：278-282
DOI：10.1016/j.mex.2015.05.006
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractSeveral chemicals have been applied in the process of coagulant extraction from herbal seeds, and the best extraction has been obtained in the presence of KCl or NaNO3[1–3], and NaCl [4]. However, the main challenge posed to these methods of coagulant extraction is their relatively low efficiency for water treatment purposes and the formation of dissolved organic matter during the treatment process. In these methods the salts, which have a one-valance metal (Na+and K+), are deposited in the internal structure and the pore of the coagulant, and may be useful for the coagulation/flocculation process. In this research, we found that modified methods produced more dense protein. Therefore, the modified procedure was better than the older one for removal of turbidity and harness from the contaminated water. Here we describe a method where:•According to the Hardy–Schulze rule, we applied the Fe3+ions instead of Na+and K+for the extraction of protein fromPlantago ovataseeds.•The method was narrowed to extract protein by ethanol (defatting) and ammonium acetate and CM-Sepharose (protein extraction).•Two consecutive elutriations of crude extract was directly performed using 0.025-M FeCl3and 0.05-M FeCl3according to the basis of the ion-exchange processes.
关键词：Water turbidity coagulant extracted from Plantago ovata seeds by using an FeCl3-induced crude extract Keywords: Plantago ovata seeds;Protein;Extraction;Coagulant