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标题：Arable agriculture changes soil microbial communities in the South African Grassland Biome
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作者：Gilbert Kamgan Nkuekam ; Don A. Cowan ; Angel Valverde 等
期刊名称：South African Journal of Science
印刷版ISSN：0038-2353
电子版ISSN：1996-7489
出版年度：2018
卷号：114
期号：5-6
页码：77-83
DOI：10.17159/sajs.2018/20170288
语种：English
出版社：The Foundation for Research Development
摘要：Many studies, mostly in temperate regions of the northern hemisphere, have demonstrated that agricul-tural practices affect the composition and diversity of soil microbial communities. However, very little is known about the impact of agriculture on the microbial communities in other regions of the world, most particularly on the African continent. In this study, we used MiSeq amplicon sequencing of bacterial 16S rRNA genes and fungal ITS regions to characterise microbial communities in agricultural and natural grassland soils located in the Mpumalanga Province of South Africa. Nine soil chemical parameters were also measured to evaluate the effects of edaphic factors on microbial community diversity. Bacterial and fungal communities were significantly richer and more diverse in natural grassland than in agricultural soils. Microbial taxonomic composition was also significantly different between the two habitat types. The phylum Acidobacteria was significantly more abundant in natural grassland than in agricultural soils, while Actinobacteria and the family Nectriaceae showed the opposite pattern. Soil pH and phosphorus significantly influenced bacterial communities, whereas phosphorus and calcium influenced fungal communities. These findings may be interpreted as a negative impact of land-use change on soil microbial diversity and composition.Significance:• This report is the first of the effect of land-use changes on the diversity of the soil microbial communities in African grassland soils.• Land-use changes influence the diversity and structure of soil microbial communities in the Grassland Biome of South Africa.• This study serves as a baseline for future studies on South African soil microbial diversity.
关键词：land use;grassland;agriculture;microbial diversity;next-generation sequencing