文章基本信息

标题：ISOLATION AND CHARACTERIZATION OF ALKALI-TOLERANT CRUDE OIL DEGRADING BACTERIA CONSORTIA FROM THE OILY SLUDGE
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作者：Yunyun Jiang ; Kelin Tao ; Chengtun Qu 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2017
卷号：26
期号：12
页码：7122-7127
语种：English
出版社：PSP Publishing
摘要：This study details the indigenous oil-degrading consortia, using crude oil as sole carbon and energy sources, which were screened out from the oily sludge of Central-Asia arid region via a crude oil mineral salt medium (MSM) plate tests. The enriched consortia are considered as heterotrophic alkali-tolerant microorganisms, which readily grew under the pH 6.0-12.0. Moreover, petroleum hydrocarbon degradation in water exhibited that the decomposition rate was the highest at pH 11.0 and reached 68.57% for the 8 d period. For the alkaline oily sludge with pH of ~10, the selected consortia could consume 60% of petroleum hydrocarbon in 7 days of aerobic cultivation, indicating that it had the potential practical application in remediation of crude oil-contaminated saline-alkaline soil and sludge. GC-MS analysis of the residual hydrocarbons showed that chain saturated alkane (C8 to C20) was greater than 90% treated for 8 days. Based on 16S rRNA gene sequences, the consortia were identified as Porphyrobactercryptus, Staphylococcus ep-idermidis, and Pseudomonas aeruginosa. Overall, the obtained oil-degrading strain presented a short growth period, the ease of isolation and screening, as well as the high crude oil degradation capacity and alkali-tolerant property, which would endow it to degrade crude-oil under alkali-wastewater or sludge.
关键词：Petroleum biodegradation;consortia screening;alkali-tolerant property;16S rRNA