文章基本信息

标题：OPTIMIZATION EXTRACTION OF LUTEOLIN IN PEONY SEED COATS
本地全文：下载
作者：Qing-huan Meng ; Yuan-gang Zu ; Hua Wang 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2017
卷号：26
期号：11
页码：6660-6667
语种：English
出版社：PSP Publishing
摘要：Enzyme assisted ethanol extraction of luteolin in peony episperm was optimized in this investigation. The effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, en-zymolysis time, ethanol concentration, solid-to-liq-uid ratio, extraction temperature, extraction time on the extraction rates of luteolin were explored by single-factor and orthogonal test. The results showed that the optimal extraction conditions were after en-zyming pretreatment in the conditions of pectinase content 0.05 mg mL"1, enzymolysis temperature 45〇C, enzymolysis pH 4.5，enzymolysis time 180 min, then 80% ethanol to extract for 2 times with extraction time 4min and liquid-to-solid ratio 10:1, luteolin extraction was up to 3.241 mg g"1. Compared with the traditional ethanol extraction, the luteolin yield was improved by 4.3 times.
关键词：Peony episperm;Luteolin;Cellulase;Pectinase