文章基本信息

标题：Distribution of Pseudomonas species in a dairy plant affected by occasional blue discoloration
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作者：Francesco Chiesa ; Sara Lomonaco ; Daniele Nucera 等
期刊名称：Italian Journal of Food Safety
电子版ISSN：2239-7132
出版年度：2014
卷号：3
期号：4
DOI：10.4081/ijfs.2014.1722
语种：Italian
出版社：PAGEPress Publications
摘要：During 2010 many cases of discoloration in mozzarella, popularly termed as blue mozzarella , have been reported to the attention of public opinion. Causes of the alteration were bacteria belonging to the genus Pseudomonas . The strong media impact of such cases has created confusion, not only among consumers, but also among experts. In order to help improving the knowledge on microbial ecology of this microorganism a study has been set up with the collaboration of a medium-sized dairy plant producing fresh mozzarella cheese, with occasional blue discoloration, conducting surveys and sampling in the pre-operational, operational and post-operational process phase, milk before and after pasteurization, water (n=12), environmental surfaces (n=22) and the air (n=27). A shelf life test was conducted on finished products stored at different temperatures (4-8°C). Among the isolates obtained from the microbiological analysis of the samples, 60 were subjected to biomolecular tests in order to confirm the belonging to Pseudomonas genus and to get an identification at species level by the amplification and sequencing of the gyrB gene. The results of microbiological tests demonstrated the presence of microorganisms belonging to the genus Pseudomonas along the entire production lane; molecular tests showed 7 different species among the 40 isolates identified. One particular species ( Pseudomonas koreensis ) was isolated from blue discolored mozzarella cheese and was indicated as the most relevant for the production plant, both for the distribution along the processing chain and for the consequences on the finished product.
关键词：Pseudomonas; Dairy products; Blue discoloration