文章基本信息

标题：Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results
本地全文：下载
作者：Danilo Pitardi ; Marzia Pezzolato ; Guia Richelmi 等
期刊名称：Italian Journal of Food Safety
电子版ISSN：2239-7132
出版年度：2013
卷号：2
期号：1
页码：12
DOI：10.4081/ijfs.2013.e12
语种：English
出版社：PAGEPress Publications
摘要：17β-estradiol is the most powerful substance with estrogenic effect, commonly used as illegal growth promoter in livestock production. To avoid health risks for consumers, sensitive, reliable and low-cost methods for quantification of extremely low concentrations of such carcinogenic residues in food are needed. Antibody-immobilised microcantilever resonators were proposed as innovative biosensors able to quantify an adsorbed target mass thanks to a shift in resonance frequency. Furthermore, the quantification of masses on the order of few picograms has recently shown to be successfully achievable with very high precision. In this study, we analysed the performance of our microcantilever sensors using extracted samples of bovine muscle from experimental animals, containing variable concentrations of 17β-estradiol (HPLC-MS/MS tested). Preliminary data showed that treated animals are correctly revealed, exhibiting large negative frequency shifts. More experiments, though, are needed to obtain a correct quantification of 17β-estradiol concentration.
关键词：17β-Estradiol detection; Bovine muscle; Microcantilever; Biosensor