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标题：Cytogenotoxicity evaluation of two industrial effluents using Allium cepa assay
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作者：Olusegun B. Samuel ; Fidelia I. Osuala ; Peter G.C. Odeigah 等
期刊名称：African Journal of Environmental Science and Technology
电子版ISSN：1996-0786
出版年度：2010
卷号：4
期号：1
页码：21-27
DOI：10.5897/AJEST09.138
语种：English
出版社：Academic Journals
摘要：The cytogenotoxic effects of the industrial effluents from paint (0, 7.2, 18, 36 and 72%) and textile (0, 1.6, 4, 8 and 16%) manufacturing were evaluated using root tip cells ofAllium cepa. In this study, root length and chromosomal aberration assays were used to determine the 96 h effective concentration (96 h EC50), root growth inhibition, mitotic index and chromosome aberration rate. Based on the 96 h EC50, textile effluent was 4.5 times more toxic than the paint effluent. Analysis of Variance (ANOVA) showed that there was significant difference (P < 0.05) in the mean root length ofA. cepaexposed to different concentrations of the industrial effluents. This indicated that the root growth inhibition was concentration dependent. The mitotic index (MI) decreased with increasing concentrations of paint and textile industrial effluents. The two industrial effluents induced chromosomal aberrations in root tip cells ofA. cepawith vagrant chromosome, bridges and fragments and sticky chromosomes being most frequently observed. At lower concentrations bridges and fragments were the most common aberration. The suitability ofA. cepachromosomal assay as a tool for monitoring the genotoxic effects of industrial effluents and wastewater is discussed.
关键词：Genotoxicity; paint; textile; industrial effluents;Allium cepa; mutation; pollution; chromosomal aberration