文章基本信息

标题：Optimization of Streptomyces sp.A11 Medium Cultivation on Cyclo(Tyrosyl-Prolyl) Production Using the Response Surface Methodology
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作者：Rofiq Sunaryanto
期刊名称：International Journal of Waste Resources
电子版ISSN：2252-5211
出版年度：2012
卷号：2
期号：2
页码：1-5
DOI：10.4172/2252-5211.1000110
语种：English
出版社：OMICS International
摘要：The Response Surface Methodology is a three factorial design which provides the relationship between one or more measured dependent response and a number of input (independent) factors. Response surface methodology was used for optimization of fermentation medium for antibacterial agent cyclo(tyr-pro) production by Streptomyces sp. A11. The optimal response region of the significant factor was predicted by using a second order polynomial model fitted to the results obtained by applying the Central Composite Design (CCD) statistical design. Determination of the best production medium of cyclo(tyr-pro) showed among 6 carbon sources used, dextrin produced the highest cyclo(tyr-pro). Among the five nitrogen sources used, peptone produced the highest cyclo(tyr-pro), and the mineral salts group I produced the highest cyclo(tyr-pro) compared with others. The results of optimization showed that three variables (dextrin as a carbon source, peptone as nitrogen source, and mixture of mineral salts) used showed significantly affected to the activity of cyclo(tyr-pro). The mathematical model used, obtained by the composition of the three variables most optimum were concentration of dextrin was 32.55 g.L-1 , peptone concentration was 11.22 g L-1 , and 8.65 mL of mineral salts. By using the composition of mathematic model, cyclo(tyr-pro) was obtained 51.54 mg L-1 (model expectations) and 50.04 mg L-1 from experiments. The differences of experiment with expectation response value was 2.91%. Using the optimized parameters, the studies demonstrated an increase in the cyclo(tyr-pro) was 2.5 fold from 20 mg L-1 to 50 mg L-1
关键词：response surface methodology; cyclo(tyrosyl-prolyl); Streptomyces sp. A11; optimization