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标题：AKTIVITAS ANTIBAKTERI PROTEIN KAPANG Xylaria psidii KT30 TERHADAP Escherichia coli DAN Bacillus subtilis [Antibacterial Activity of Protein Fungus Xylaria psidii KT30 Escherichia coli and Bacillus subtilis]
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作者：Aris Munandar ; A. Zaenal Mustopa ; Kustiariyah Tarman 等
期刊名称：Jurnal Teknologi Dan Industri Pangan
印刷版ISSN：2087-751X
出版年度：2014
卷号：25
期号：2
页码：146
DOI：10.6066/9104
语种：English
出版社：Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI
摘要：Previous research shows that extracellular protein of an algicolous fungus Xylaria psidii KT30 inhibited Bacillus pumilus, Listeria sp., Salmonella typhi, Staphylacoccus aureus, and Pseudomonas sp. with an average clear zone diameter of 7 mm. To enhance the potent antibacterial activity of extracellular protein from Xylaria psidii KT30, t he present research demonstrated fungal growth optimization and purification of its secreted extracellular protein. T he fungal growth optimization was performed following NaCl concentration and harvest day variations . The protein was p recipitat ed using ammonium sulphate at 60%-90% saturation range and was purifi ed through gel chromatography filtration using Sephadex G-50, eluted with 30% aq methanol . The active fraction possesing a ntibacterial activit y was then determined resulting supernatant, pellet, and protein fraction. The fungal was optimally obtained after 15 days cultivation using freshwater.The highest protein yield was 1.67% , resulted over 90% saturation. Fractions 11 and 12 were the most active against Escherichia coli dan Bacillus subtilis with clear zone diameter of 8 mm. Three bands of those fractions were detected through SDS-PAGE analysis, revealing molecular weights of 23.42, 20.09 , and 14.33 kDa.
关键词：antibacterial;marine fungi;protein purification;Xylaria psidii