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  • 标题:Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing
  • 本地全文:下载
  • 作者:Alexander Gorel ; Koji Motomura ; Hironobu Fukuzawa
  • 期刊名称:Scientific Data
  • 电子版ISSN:2052-4463
  • 出版年度:2017
  • 卷号:4
  • DOI:10.1038/sdata.2017.188
  • 语种:English
  • 出版社:Nature Publishing Group
  • 摘要:We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.
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