文章基本信息

标题：Effect of Ethyl Methanesulfonate on Fatty Acid Characteristic of Nannochloropsis sp.
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作者：Dina Augustine ; Mujizat Kawaroe ; Agus Oman Sudrajat 等
期刊名称：International Journal of Environment and Bioenergy
电子版ISSN：2165-8951
出版年度：2014
卷号：9
期号：3
页码：196-204
语种：English
出版社：Modern Scientific Press
摘要：The ability of microalgae to grow in short time with huge biomass production has become the reference point to produce biofuel in short time as well. Until now, it still needs microalgae species that is capable of producing numerous biomass and high levels of fatty acid so it is able to supply basic needs for biofuel. One of the method used to increase microalgae biomass is mutagenesis. Mutagenesis method here is conducted by adding certain compounds called Ethyl Methanesulfonate (EMS) to microalgae cell. The results of this study showed that the highest biomass was obtained from Nannochloropsis sp. cultivation with 0.1 M EMS concentration in two different phases (1.08 ± 0.33 and 1.11 ± 0.07 gr/L). Gas chromatography identification has detected the presence of 22 compounds of saturated fatty acid and 3 compounds of unsaturated fatty acid. Three compounds of unsaturated fatty acid detected were C18:2 cis-9 (methyl oleate), C16:1 (methyl palmitoleate) and C18:2 (methyl linoleic). The highest percent area/concentration of saturated and unsaturated fatty acid respectively is C16:0 (methyl palmitate) as much as 67.70±0.05 at stationary phase and C16:1 (methyl palmitoleate) as much as 17.59±0.42 at death phase. From the results, it can be implied that EMS input in microalgae cultivation can increase the percentage of certain fatty acids that potentially produce to be biodiesel.
关键词：Nannochloropsis sp.;mutagenesis;ethyl methanesulfonate;biomass;fatty acid