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标题：Bioconversion Studies of Methyl Laurate to Dodecanedioic Acid using a Wild-type of Candida tropicalis
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作者：Rifkah Akmalina ; Ronny Purwadi ; Johnner Sitompul 等
期刊名称：MATEC Web of Conferences
电子版ISSN：2261-236X
出版年度：2018
卷号：156
DOI：10.1051/matecconf/201815601001
语种：English
出版社：EDP Sciences
摘要：Production of dodecanedioic acid (DDDA), a platform chemical used as raw material for various commodities and polymers, has been studied through a biological process. This process was conducted by using a wild-type ofCandida tropicaliswhich can be obtained easily from natural resources. The aim of this research was to study the characteristics of DDDA production from methyl laurate through batch fermentation process. Growth phase was carried out for 20 h, as the beginning of fermentation, then continued to conversion phase for 5 until 6 days. Utilization of methyl laurate and production of DDDA were analysed using gas chromatography, which proved the ability ofC. tropicalisin assimilating methyl laurate to convert it become DDDA. The highest value of cells yield (Yx/s) and DDDA yield (Yp/s) successfully obtained were 0.86 g cells/g methyl laurate and 0.20 g DDDA/g methyl laurate, respectively. This study also showed the possibility of fermentation products accumulation as intermediate, or accumulation of DDDA inside the cells. Thus, this study can be applied as an alternative in addition to the use of mutant microorganism in producing DDDA.