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标题：Genotoxicity of Titanium Dioxide Nanoparticles using the Mouse Bone Marrow Micronucleus and Sperm Morphology Assays
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作者：Bakare AA ; Udoakang AJ ; Anifowoshe AT 等
期刊名称：Journal of Pollution Effects & Control
电子版ISSN：2375-4397
出版年度：2016
卷号：4
期号：2
DOI：10.4172/2375-4397.1000156
语种：English
出版社：OMICS International
摘要：Titanium dioxide nanoparticles (TiO2-NPs) have recently been of public health and scientific concern due to their widespread use in industrial and household applications. However, there is limited information concerning its in vivo cytogenotoxicity. In this study, the cytogenotoxic effects of TiO2-NPs on the somatic tissue using the mouse bone marrow micronucleus (MN) assay and on reproductive tissue using the mouse sperm morphology assay and testicular histopathology were investigated. Five concentrations of 9.38, 18.75, 37.50, 75.00 and 150.00 mg/kg bwt were administered intraperitoneally at 0.5 mL/mouse to mice for five and ten consecutive days in the MN assay; and for five consecutive days in the sperm morphology assay. Double distilled water and cyclophosphamide (20 mg/kg bwt) served as negative and positive controls, respectively. A significant (p<0.05) increase in MN was observed in bone marrow cells of treated mice at 37.50 mg/kg bwt concentration in the 5-day exposure and at all concentrations in the 10-day exposure. The sperm cells examined 5 and 10 weeks from the first day of exposure showed significant increase (p<0.05) in abnormal sperm cells at tested concentrations. Histopathologically, TiO2-NPs disrupted the normal cellular architecture of testicular tissues in exposed mice; as it caused severe lesions such as congestion of the interstitium oedema, vacuolation and necrosis. These suggest that the bone marrow and testicular cells may be potential targets for TiO2-NPs induced DNA damage and cytotoxicity in mice. This is of public health importance considering increasing exposure to TiO2-NPs in consumer products.
关键词：Titanium dioxide nanoparticles; DNA damage; Micronucleus; Histopathology; Mouse sperm morphology