文章基本信息

标题：A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS
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作者：Stanislav Naryzhny ; Victor Zgoda ; Artur Kopylov 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2017
卷号：4
页码：260-264
DOI：10.1016/j.mex.2017.08.004
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractA method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied.After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip was cut to sections, and every section was treated according to trypsinolysis protocol for MS/MS analysis. The peptides produced were further analyzed by ESI LC-MS/MS. The procedure allows to:•identify many more proteins and proteoforms compared to shotgun analysis of extracts.•build a semi-virtual 2DE map of identified proteins.
关键词：Collective states Shape phase transition Critical point Shape coexistence Sextic potential