文章基本信息

标题：Metabolic profiling of body fluids and multivariate data analysis
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作者：Jean-Pierre Trezzi ; Christian Jäger ; Sara Galozzi 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2017
卷号：4
页码：95-103
DOI：10.1016/j.mex.2017.02.004
语种：English
出版社：Elsevier
摘要：Graphical abstractQuantitative multi-component analysis of body fluids with GC–MS. After sample collection, the samples are pre-processed and used for metabolite extraction. After this, GC–MS analysis and subsequent data processing and analysis are performed.Display OmittedAbstractMetabolome analyses of body fluids are challenging due pre-analytical variations, such as pre-processing delay and temperature, and constant dynamical changes of biochemical processes within the samples. Therefore, proper sample handling starting from the time of collection up to the analysis is crucial to obtain high quality samples and reproducible results. A metabolomics analysis is divided into 4 main steps: 1) Sample collection, 2) Metabolite extraction, 3) Data acquisition and 4) Data analysis.Here, we describe a protocol for gas chromatography coupled to mass spectrometry (GC–MS) based metabolic analysis for biological matrices, especially body fluids. This protocol can be applied on blood serum/plasma, saliva and cerebrospinal fluid (CSF) samples of humans and other vertebrates. It covers sample collection, sample pre-processing, metabolite extraction, GC–MS measurement and guidelines for the subsequent data analysis.Advantages of this protocol include:•Robust and reproducible metabolomics results, taking into account pre-analytical variations that may occur during the sampling process•Small sample volume required•Rapid and cost-effective processing of biological samples•Logistic regression based determination of biomarker signatures for in-depth data analysis
关键词：Metabolomics;Metabolite extraction;Blood;Saliva;Cerebrospinal fluid;GC–MS;Data analysis;Body fluids