文章基本信息

标题：Rapid biodegradation of polycyclic aromatic hydrocarbons (PAHs) using effective Cronobacter sakazakii MM045 (KT933253)
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作者：Zubairu Darma Umar ; Nor Azwady Abd. Aziz ; Syaizwan Zahmir Zulkifli 等
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2017
卷号：4
页码：104-117
DOI：10.1016/j.mex.2017.02.003
语种：English
出版社：Elsevier
摘要：Graphical abstractDisplay OmittedAbstractPolycyclic Aromatic Hydrocarbons (PAHs) are complex and widely distributed environmental pollutants that can affect living ecosystems. This study was conducted to rapidly degrade phenanthrene and pyrene representing low and high molecular weight of PAHs, respectively.Cronobacter sakazakiiMM045 (KT933253) was identified from used engine oil of contaminated soil. PAHs biodegradation was carried out using 2,6-dichlorophenol indophenol (DCPIP) assay. Biodegradation influencing factors including agitation, temperature, pH, inoculums volume and salinity were enhanced using Response Surface Methodology (RSM) by Central Composite Design (CCD). Phenanthrene and pyrene biodegrading metabolites were identified using gas chromatography mass spectrophotometer (GCMS).•Initial biodegradation indicated 75.2% and 54.3% phenanthrene and pyrene degraded byC. sakazakiiMM045 within 24h. After CCD optimisation, 100% degradation was achieved for each of the phenanthrene and pyrene, resulting in the formation of intermediate metabolites.•The identified phenanthrene metabolites were 3,4-dihydroxyphenathrene, phthalic acid, pyruvic acid, acetic acid and oxalic acid. Pyrene intermediates comprised pyrenecis-4,5-dihydrodiol, 3,4-dihydroxyphenanthrene, phthalic acid, pyruvic acid, acetic acid and lactic acid.•Cronbacter sakazakiiMM045 was proven to be rapid and effective in degrading PAHs within 24h despite the unavailability of existing literatures on PAHs biodegradation.
关键词：Biodegradation;Phenanthrene;Pyrene;Optimization;C. sakazakii MM045