文章基本信息

标题：Field detection of proteins by a disposable strip
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作者：Baoshan He ; Huali Jin ; Mannan Li 等
期刊名称：Scientific Research and Essays
印刷版ISSN：1992-2248
出版年度：2011
卷号：6
期号：7
页码：1688-1691
DOI：10.5897/SRE10.059
语种：English
出版社：Academic Journals
摘要：A novel disposable strip which included filtration membrane, reaction film, absorbent pad, substrate and plastic card was designed to detect proteins on site. Protein samples percolated through the filtration membrane, a protein-dye complex was generated by the quick binding reaction between the passed protein and the dye Coomassie Brilliant Blue G-250 which was adsorbed in the reaction film. Meanwhile, the film color turned from brownish red to blue. The deeper blue film was obtained in an increased protein concentration-dependent manner. Experimental results demonstrated that the protein concentrations were proportional to b values of the L a b color system. A favorable linearity was presented in the range of 0.1 to 1000 μg/ml, and the correlation coefficient (r) was -0.987. The whole testing time needed was about in 2 min at room temperature, and the strip could meet the need of the field detection of proteins.
关键词：Protein; disposable strip; field detection