文章基本信息

标题：Laser raman and infrared spectrum analysis on low-density lipoproteins purified from hen egg yolk
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作者：Xue Hui-jun ; Li Sheng ; Wang Hua 等
期刊名称：Scientific Research and Essays
印刷版ISSN：1992-2248
出版年度：2012
卷号：7
期号：33
页码：2962-2965
DOI：10.5897/SRE11.1965
语种：English
出版社：Academic Journals
摘要：Diversified proteins were separated from hen egg yolk by ammonium sulphate rapid fractionation, and pure low-density lipoprotein (LDL) was obtained after filtrating through Sephadex G-200 chromatography. After the qualitative detection ofSodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), we discovered that LDL consists of five major apoproteins. The results show that: (1) the CH2 presents asymmetric stretching and symmetric stretching mode, (2) thederivative of cholesterol C=C stretching vibration is at 1652.07 cm-1 in the Raman spectrum, and (3) a-helix bands in apolipoprotein is at 1655.12 cm-1 in the infrared spectrum. The results also suggest that laser raman and infrared spectrum analysis on LDL can provide useful information for studying the LDL structure.
关键词：Low-density lipoprotein (LDL); sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); C-H stretching vibration; infrared spectrum; laser raman spectrum