文章基本信息

标题：Isolation of Bacillus sphaericus screening larvicidal, fecundity and longevity effects on malaria vector Anopheles stephensi
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作者：A. Naresh kumar ; K. Murugan ; K. Shobana 等
期刊名称：Scientific Research and Essays
印刷版ISSN：1992-2248
出版年度：2013
卷号：8
期号：11
页码：425-431
DOI：10.5897/SRE10.373
语种：English
出版社：Academic Journals
摘要：In India, the most important diseases transmitting mosquitoes belong to the genera Anopheles, Culex, Aedes and Mansonia. The indiscriminate use of synthetic insecticides creates multifarious problems like environmental pollution, global warming, insecticide resistance and is hazardous to humans. In the present study, bacterial pesticide, Bacillus sphaericus a soil dwelling bacteria, isolated from the sludge samples collected from Bharathiar University, Coimbatore, India cultured with required substrates and used to control the malaria vector, Anopheles stephensi. Bioassay was conducted to test the larval toxicity, fecundity and mosquito longevity. LC50 and LC90 values were calculated for the larval instars of the malaria vector, A. stephensi. The fecundity and adult longevity were highly reduced after the treatment with B. sphaericus. Field trials were also conducted in different breeding sites of A. stephensi with different range of temperature and altitude. Percentage reduction of larval population was maximum (100%) at 96 h of treatment. Toxicity efficacy of B. sphaericus was varied according to the temperature and altitude of the study area. The bacterial pesticide B. sphaericusproved that it is an effective mosquito control agent and can be used for further integrated pest management programmes.
关键词：Bacillus sphaericus; larval toxicity; lethal concentration; Anopheles stephensi; field studies