文章基本信息

标题：Molecular diagnosis of avian respiratory diseases in commercial broiler chicken flocks in province of Najaf, Iraq
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作者：Ali M. Al-Mohana ; Haider M. Kadhimv ; Alaa H. Al-Charrakh 等
期刊名称：Scientific Research and Essays
印刷版ISSN：1992-2248
出版年度：2013
卷号：8
期号：26
页码：1191-1195
DOI：10.5897/SRE11.816
语种：English
出版社：Academic Journals
摘要：Avian influenza virus subtype 9 and Newcastle virus have been recognized as the most important pathogens in poultry. In Najaf governorate, Iraq, many chickens’ flocks suffered from high mortality (about 30 to 70%) and respiratory signs. In this study, trachea swabs and tissue specimens from 53 commercial broiler chicken flocks suffering from respiratory diseases were tested initially by rapid test for antigen influenza type A and by reverse transcription polymerase chain reaction (PCR) for avian flu virus type A, H5, H7, H9, and Newcastle disease virus (NDV). The reverse transcription PCR results showed that 75% of these flocks were infected with both avian flu virus type H9 and NDV whereas 25% of them were infected only by H9. On the other hand, all flocks were negative for subtypes H5 and H7. Our data showed that aforementioned respiratory pathogens were the most important agents of respiratory diseases giving high mortality in broiler chicken in the area of the study.
关键词：Respiratory diseases; avian flu; broiler chicken; molecular diagnosis; Iraq