文章基本信息

标题：Up-Streaming Process for Glucose Oxidase by Thermophilic Penicillium sp. in Shake Flask
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作者：Muhammad Mohsin JAVED ; Aroosh SHABIR ; Sana ZAHOOR 等
期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2012
卷号：9
期号：2
页码：127-133
DOI：10.2004/wjst.v9i2.216
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp . in 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. Effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. Maximum yield (0.325 U/mL) of extra-cellular glucose oxidase was obtained after 72 hrs of incubation at 45 o C using submerged fermentation technique in shaking conditions utilizing sucrose as carbon source.
关键词：Glucose oxidase, thermophilic, Penicillium, submerged fermentation