文章基本信息

标题：Development of Insertion and Deletion Markers for Bottle Gourd Based on Restriction Site-associated DNA Sequencing Data
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作者：Xinyi WU ; Pei XU ; Xiaohua WU 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2017
卷号：3
期号：1
页码：13-16
DOI：10.1016/j.hpj.2017.01.011
语种：English
出版社：Elsevier
摘要：Bottle gourd is an important cucurbit crop worldwide. To provide more available molecular markers for this crop, a bioinformatic approach was employed to develop insertion–deletions (InDels) markers in bottle gourd based on restriction site-associated DNA sequencing (RAD-Seq) data. A total of 892 Indels were predicted, with the length varying from 1 bp to 167 bp. Single-nucleotide InDels were the predominant types of InDels. To validate these InDels, PCR primers were designed from 162 loci where InDels longer than 2 bp were predicated. A total of 112 InDels were found to be polymorphic among 9 bottle gourd accessions under investigation. The rate of prediction accuracy was thus at a high level of 72.7%. DNA fingerprinting for 4 cultivars were performed using 8 selected Indels markers, demonstrating the usefulness of these markers.
关键词：bottle gourdInDels markersRAD-SeqDNA fingerprinting