文章基本信息

标题：Application of Dynamic Metabolic Flux Convex Analysis to CHO-DXB11 Cell Fed-batch Cultures
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作者：Sofia Fernandes ; Julien Robitaille ; Georges Bastin 等
期刊名称：IFAC PapersOnLine
印刷版ISSN：2405-8963
出版年度：2016
卷号：49
期号：7
页码：466-471
DOI：10.1016/j.ifacol.2016.07.386
语种：English
出版社：Elsevier
摘要：In this work, a dynamic metabolic flux analysis based on convex analysis (DMFCA) is applied to CHO-DXB11 cell fed-batch cultures. This approach exploits all the available knowledge of the metabolic network and the time evolution of extracellular component concentrations, to determine bounded intervals for the fluxes continuously over time. Smoothing splines and mass balance differential equations are used to estimate the time evolution of the uptake and excretion rates from experimental data. Furthermore, the method is suitable for underdetermined systems, and does not require the definition of ad-hoc objective functions to be optimized. Moreover the metabolic network considered in this work allows an estimation of the carbon dioxide flux.
关键词：CHO cellsMetabolic networkDynamic metabolic flux analysisUnderdetermined systemConvex analysisMathematical modeling