文章基本信息

标题：Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy
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作者：Yiran Ren ; Qiang Zhao ; Xianyan Zhao 等
期刊名称：Horticultural Plant Journal
印刷版ISSN：2468-0141
出版年度：2016
卷号：2
期号：2
页码：61-66
DOI：10.1016/j.hpj.2016.06.007
语种：English
出版社：Elsevier
摘要：A bHLH transcription factor that is induced by low temperature was found in apple (Malus × domestica Borkh.). To understand the sequence characteristics of the gene, bioinformatics analysis was performed. Furthermore, gene expression patterns of the laminated apple seeds and lateral flower buds were analyzed during the period of dormancy release with semi-quantitative RT-PCR. Based on secondary structure predictions, the results showed that the MdCIbHLH1 protein structure mainly included α-helix and random coil, while β-sheet and extended strand content was less. Semi-quantitative RT-PCR analysis showed that the expression patterns of MdCIbHLH1 were similar in laminated apple seeds and lateral flower buds during the period of dormancy release. Before dormancy release, expression levels of MdCIbHLH1 were high and gradually decreased during the period of dormancy release. These results indicated that MdCIbHLH1 might play an important role during dormancy release in apple seeds and apple buds.
关键词：apple MdCIbHLH1 dormancyexpression analysis