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标题：NGS-based deep bisulfite sequencing
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作者：Suman Lee ; Joomyeong Kim
期刊名称：MethodsX
印刷版ISSN：2215-0161
电子版ISSN：2215-0161
出版年度：2016
卷号：3
页码：1-7
DOI：10.1016/j.mex.2015.11.008
语种：English
出版社：Elsevier
摘要：Graphical abstract Display Omitted Abstract We have developed an NGS-based deep bisulfite sequencing protocol for the DNA methylation analysis of genomes. This approach allows the rapid and efficient construction of NGS-ready libraries with a large number of PCR products that have been individually amplified from bisulfite-converted DNA. This approach also employs a bioinformatics strategy to sort the raw sequence reads generated from NGS platforms and subsequently to derive DNA methylation levels for individual loci. The results demonstrated that this NGS-based deep bisulfite sequencing approach provide not only DNA methylation levels but also informative DNA methylation patterns that have not been seen through other existing methods. • This protocol provides an efficient method generating NGS-ready libraries from individually amplified PCR products. • This protocol provides a bioinformatics strategy sorting NGS-derived raw sequence reads. • This protocol provides deep bisulfite sequencing results that can measure DNA methylation levels and patterns of individual loci.
关键词：DNA methylation;Bisulfite sequencing;Epigenomes;NGS