文章基本信息

标题：A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma
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作者：Srinivasa Reddy ; Srinivasa Reddy ; Licto Thomas 等
期刊名称：Journal of Analytical Science and Technology
电子版ISSN：2093-3371
出版年度：2016
卷号：7
期号：1
页码：1-10
DOI：10.1186/s40543-016-0097-2
语种：English
出版社：Korea Basic Science Institute
摘要：Abstract Background A new LC–MS/MS method for the simultaneous determination of lamivudine, zidovudine, and nevirapine in human plasma is developed using column-coupling technique. Method Labeled compound of respective analyte was used as an internal standard. After extraction from 100 μL plasma by solid phase extraction method, analytes were separated on a C18 column coupled with a cation exchange column. Total run time was 4.5 min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring under positive ionization mode with an electrospray ionization interface. The method was validated as per the FDA guidelines over the concentration range of 9.47–1466.67 ng/mL for lamivudine, 10.32–1600.00 ng/mL for zidovudine, and 15.05–2426.67 ng/mL for nevirapine. Results Precision was in the range 0.86–5.77 (intraday) and 1.92–8.19 (interday) while accuracy was 93.25–104.36 % (intraday) and 96.83–103.28 % (interday). Stabilities of stock in aqueous solutions and in plasma were also determined. Conclusion The method can be applied to the pharmacokinetic study of a combination treatment.