文章基本信息

标题：Controlling time scales for electron transfer through proteins
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作者：Scot Wherland ; Scot Wherland ; Israel Pecht 等
期刊名称：Perspectives in Science
印刷版ISSN：2213-0209
电子版ISSN：2213-0209
出版年度：2015
卷号：6
页码：94-105
DOI：10.1016/j.pisc.2015.06.005
语种：English
出版社：Elsevier
摘要：Summary Electron transfer processes within proteins constitute key elements in biological energy conversion processes as well as in a wide variety of biochemical transformations. Pursuit of the parameters that control the rates of these processes is driven by the great interest in the latter reactions. Here, we review a considerable body of results emerging from investigation of intramolecular electron transfer (ET) reactions in two types of proteins, all done by the use of the pulse-radiolysis method: first are described results of extensive studies of a model system, the bacterial electron mediating protein azurin, where an internal ET between the disulfide radical ion and the Cu(II) is induced. Impact of specific structural changes introduced into azurin on the reaction rates and the parameters controlling it are discussed. Then, the presentation is extended to results of investigations of intra-protein ET reactions that are part of catalytic cycles of multi-copper containing enzymes. Again, the rates and the parameters controlling them are presented and discussed in the context of their efficacy and possible constraints set on their evolution.
关键词：Copper proteins;Multicopper oxidases;Azurin;Activation parameters;Marcus theory;Pulse radiolysis