文章基本信息

标题：Assessment of biofilm formation by enterococci isolates from urinary tract infections with different virulence profiles
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作者：Hossein Samadi Kafil ; Hossein Samadi Kafil ; Ashraf Mohabati Mobarez 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2015
卷号：27
期号：4
页码：312-317
DOI：10.1016/j.jksus.2014.12.007
语种：English
出版社：Elsevier
摘要：Abstract This study aimed to investigate possible associations between virulence profiles and biofilm formation in Clinical UTI isolates. Isolates were collected from five university hospitals and identified and characterized for the presence of virulence factors by PCR. Biofilm assays were conducted in 96 well microtiter plates by reading the OD570 after crystal violet staining. 75% of isolates had esp gene, 38.77% had asa1, 84.18% had ace, 81.63% had efaA, 93.36% had ebpR, 34.18% had cylA, 81.63% had gelE and 17.35% had hyl. Biofilm experiences were done and isolates having asa1 or efaA genes produced more biofilms than negative ones (P =0.011, P =0.008), but the presence of esp, ace, cylA or gelE genes in isolates had no effect on biofilm formation. Isolates possessing hyl had much lower biofilm formation (P =0.000). Present study showed that the esp, ace, gelE and cylA genes do not seem to be necessary nor sufficient for the production of biofilm in enterococci but the presence of efaA and asa1 correlates with increased biofilm formation of urinary tract isolates. Also the low prevalence of hyl among enterococci isolated from UTIS and its association with poor biofilm production indicate that the absence of this gene can be an advantage in the pathogenesis of UTIs.
关键词：Enterococci; Biofilm; UTIs; Virulence genes; Colonization; Secretory factors;