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标题：Partial characterization of phenoloxidase enzyme in the hemocytes of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)
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作者：Mohsen Taleh ; Mohsen Taleh ; Mohammad Saadati 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2014
卷号：26
期号：4
页码：285-289
DOI：10.1016/j.jksus.2013.08.005
语种：English
出版社：Elsevier
摘要：Abstract Phenoloxidase (PO) activity plays a key role in the innate immune responses of insects, which catalyzes the biosynthesis of quinones and other reactive intermediates to eliminate invading pathogens and parasites. This study was conducted to characterize the biochemical properties of the PO enzyme from the hemocytes of the cotton bollworm Helicoverpa armigera Hübner. The maximum activity of PO occurred at pH 6 and at 30°C and it was stable for 12–24h. The Michaelis–Menten constant (Km ) and the maximal velocity (V max) were determined as 1.86mM and 0.35U/mg protein, respectively. This is the first report of PO characterization in H. armigera from Iran that provided biochemical optimized conditions for its activity, and with these ongoing studies, our aim will be to develop new strategies for cotton bollworm control using disruptors of the immune system.
关键词：Phenoloxidase; Helicoverpa armigera ; Hemocyte; Innate immune;