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标题：EPIYA (or -like) motifs in mammalian proteins
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作者：Fatemeh Safari ; Fatemeh Safari
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2014
卷号：26
期号：4
页码：276-284
DOI：10.1016/j.jksus.2014.05.001
语种：English
出版社：Elsevier
摘要：Abstract Recently, two mammalian proteins with functional Glu-Pro-Ile-Tyr-Ala (EPIYA) or a similar sequence (EPIYA-like motif) have been identified. Pragmin contains a single EPIYA motif and p140Cap contains two EPIYA-like motifs (EPLYA/EGLYA). Searching the human proteome using the NCBI BLAST software revealed that EPIYA-like motifs such as EPLYA, ESIYE, EDLYA, ESIYA, EHIYD, EPIYD, ENIYE, EPVYA, EVVYA, and TPLYA (which are present in bacterial effectors and have roles in virulence) are present in mammalian proteins. Moreover, several of bacterial EPIYA-like motifs were duplicated in mammalian proteins. Since, only five mammalian proteins contain EPIYA motif, and there are no mammalian proteins with more than one copy of the EPIYA motif. We also showed that the most of EPIYA (or -like) motifs in mammalian proteins are predicted to be naturally ordered. Moreover, few mammalian proteins containing EPIYA (or -like) motif were tyrosine phosphorylated. On the other hand, most of the mammalian proteins contain “unfunctional EPIYA (or -like) motifs”. Notably, regulation of Src family kinases’ (SFKs) activity (including up and down regulation) originally is mediated by their own EPIYA like motifs (EPIYI and EPQYQ) and thereby, it seems that EPIYA (or -like) motifs are “potential regulators of SFKs”. Therefore, it can explain why EPIYA (or -like) motifs are restricted sites with low copy number in most mammalian proteins whereas in bacterial effector proteins, they can be universal sites with repetitive copies. In this study, we identified new mammalian proteins which contain functional EPIYA (or -like) motifs and thereby, their possible roles in cell signal transduction pathways. In this respect, functional experiments will be necessary to confirm our findings.
关键词：EPIYA (or -like) motif; Mammalian proteins; SFKs; Bacterial effector proteins;