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标题：Melatonin exhibits antioxidant properties in a mouse brain slice model of excitotoxicity
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作者：Kimberly L. Clapp-Lilly ; Mark A. Smith ; George Perry 等
期刊名称：International Journal of Circumpolar Health
印刷版ISSN：1239-9736
电子版ISSN：2242-3982
出版年度：2002
卷号：61
期号：1
DOI：10.3402/ijch.v61i1.17403
语种：English
摘要：Objectives. Stroke is a major cause of brain injury in Alaska. Since antioxidant levels are decreased in aged brain, the greater predisposition to neuronal death in stroke leading to subsequent neurodegeneration in aged individuals may be related to changes in oxidant balance. We studied the effect of the endogenous antioxidant melatonin on excitotoxic injury resulting from N-methyl- D-aspartate (NMDA)-induced damage by developing an organo-typic mouse brain slice model. Our objective was to inhibit the effects of oxidative stress induced by NMDA in mouse brain slices, using melatonin. Methods. An organotypic mouse brain slice culture was established at PO2 levels maintained between 80 – 100 mm Hg. NMDA, melatonin or both were added to the slices and antioxidant function was determined using the redox active iron assay as well as 8-OHG and HO-1 immunoassays. Results. This slice system allows for better regulation of both NMDA and melatonin concentrations than can be achieved by in vivo studies. Supporting an antioxidant function, melatonin (100 μM) significantly decreased redox active iron, hemexygenase (HO-1) induction and 8-hydroxyguanosine (8-OHG) following NMDA (50-100 μM) insult. However, somewhat surprisingly, high concentrations of melatonin alone (1mM), increased redox active iron levels and HO-1 induction. Conclusions. These results support the hypothesis that melatonin is a neuroprotective antioxidant. Our data also suggest that 1mM melatonin may have detrimental effects.(Int J Circumpolar Health 2002; 61(1):32-40)Keywords: antioxidant, brain slices, excitotoxicity, melatonin, stroke
关键词：antioxidant, brain slices, excitotoxicity, melatonin, stroke