文章基本信息

标题：SCREENING AND CHARACTERIZATION OF BACTERIAL CHITOSANASE FROM MARINE ENVIRONMENT
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作者：Ekowati Chasanah ; Dewi Seswita Zilda ; Agustinus R Uria 等
期刊名称：JOURNAL OF COASTAL DEVELOPMENT
印刷版ISSN：1410-5217
出版年度：2009
卷号：12
期号：2
页码：64-72
语种：English
出版社：JOURNAL OF COASTAL DEVELOPMENT
摘要：Screening of extracellular chitosanase from bacterial isolates associated with marine sponges have been done. Out of 100 bacterial isolates, forty isolates were capable of forming clearing zones on the chitin media and one isolate, 34-b, produced the highest chitinolytic index. The enzymes was produced on chitin liquid medium at 37oC in a shaking waterbath for a five-day cultivation. Crude enzymes were prepared by cell-free supernatant (CFS) and concentrated through 70% (saturated) ammonium sulphate percipitation followed by dialysis. The enzymes worked best at pH and temperature of 6-7 and 60oC, respectively. The half-life (T1/2) for chitosanase activity was 500.2 min or 8.34 hours (at 37oC) and 55.12 min (at 50oC), indicating the enzyme are quite stable at that temperature. However, around 80% of the original activity was lost at 60oC after 15 min of incubation.
关键词：chitosanase, marine environment